Bioinformatics is an interdisciplinary field which concentrates on developing tools to better understand, analyze and gather information about the biological data. This field brings together mathematics, Statistics, Computer Science, Bio-Engineering, and Computer Engineering to analyze complex problems facing biologists today. Today, with large biological data gathered, it is becoming increasing hard to analyze, using old hard manual means. This is where computation help is required and over time, many computation solutions are developed to analyze and produce 'good' biological data to scientist. One can also perceive this field to be a field of data mining or data science to solve problems in Biology and Medicine. In this broad field of Bioinformatics, this book concentrates on DNA pairwise sequencing, which is an important initial step in Bioinformatics world. This book details out the fundamental concepts of Pairwise and multiple sequence alignment and move on to local and global sequence alignment algorithms